中文名称：ERK1磷酸化丝裂原活化蛋白激酶1抗体

英文名称：Anti-phospho-ERK1(Thr202/Tyr204)

别名：Erk1 (pT202/pY204); ERK/MAPK(phospho T202/Y204); ERK1 (phospho T202); p-ERK1 (phospho T202); p44/42 MAP Kinase(Phospho-Thr202); ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.  

规 格：100ul

浓    度：1mg/ml

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Guinea Pig,

产品类型：一抗  

研究领域：免疫学 神经生物学 信号转导 干细胞 激酶和磷酸酶

分子量：43kDa

性    状：Lyophilized or Liquid

免 疫 原：KLH conjugated Synthesised phosphopeptide derived from rat ERK1 around the phosphorylation site of Thr201/204:FL(p-T)E(p-Y)VA 

亚    型： IgG 

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用：ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500（石蜡切片需做抗原修复） 

ERK1磷酸化丝裂原活化蛋白激酶1抗体荧光标记抗体实验要点：

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平；

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率)；

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定；

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存；

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。

与ERK1磷酸化丝裂原活化蛋白激酶1抗体相关产品：

Lec1(卵巢细胞)     ATCC    

293T/17 [HEK 293T/17] (人胚肾细胞)     ATCC    

AAV-293( 胚肾细胞 )     ATCC    

hFOB 1.19(SV40 转染成骨细胞)     ATCC    

CCD-1095Sk(皮肤细胞 )     ATCC    

Hs 578Bst(乳腺细胞)     ATCC    

NK-92(人恶性非霍奇金患者的自然杀伤细胞)     ATCC    

NK-92MI(人恶性非霍奇金患者的自然杀伤细胞)     ATCC    

HMy2.CIR(人B 淋巴母细胞)     ATCC    

OK(负鼠(opossum)肾细胞)     ATCC    

FO(细胞)     ATCC    

RAW 264.7(单核巨噬细胞)     ATCC    

P19(畸胎癌细胞)     ATCC    

C6(细胞)     ATCC    

RBL-2H3(细胞)     ATCC    

SiHa(子细胞)     ATCC    

MG-63(细胞)     ATCC    

ERK1磷酸化丝裂原活化蛋白激酶1抗体   HEC-1-B(子宫内膜腺癌细胞)     ATCC    

SW1353(细胞)     ATCC    

ES-2(卵巢透明细胞癌细胞)     ATCC