中文名称：ENPP1核苷酸内焦磷酸酶/磷酸二酯酶1抗体

英文名称：Anti-ENPP1

别名：Alkaline phosphodiesterase 1; ARHR2; E-NPP 1; Ectonucleotide pyrophosphatase/phosphodiesterase 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ENPP1_HUMAN; Ly 41 antigen; M6S1; Membrane component chromosome 6 surface marker 1; NPP1; NPPase; NPPS; Nucleotide pyrophosphatase; PC-1; PCA1; PDNP1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1.  

规 格：100ul

浓    度：1mg/ml

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat, Dog, Pig, Horse, Rabbit

产品类型：一抗  

研究领域： 免疫学 信号转导 激酶和磷酸酶 

分子量：100kDa

性    状：Lyophilized or Liquid

免 疫 原：KLH conjugated synthetic peptide derived from human ENPP1:41-140/925 <Extracellular>

亚    型： IgG 

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 

ENPP1核苷酸内焦磷酸酶/磷酸二酯酶1抗体荧光标记抗体实验要点：

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平；

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率)；

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定；

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存；

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。

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